The MBP can assist research scientists at all skill levels in the design and preparation of transgenic and targeting constructs for generating genetically altered mice. Staff is available to consult with investigators on optimal or alternative approaches for developing transgenic and knockout mice, including selection of appropriate promoters for inducing conditional mutations. We specialize in the production of transgenic and gene-targeted mice through both conventional as well as conditional and induceable approaches. In addition to provision of technically-qualified and expert staff, we have the essential resources to screen mouse genomic libraries, sequence genomic and plasmid DNA, assemble restriction digest maps, construct and purify targeting vectors, and electroporate, select, and expand ES cell clones. These resources facilitate the efficient development of mouse models for the scientific community.